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This highly targeted approach enables a wide range of applications for discovering, validating, and screening genetic variants in a rapid and efficient manner. Requiring just 150 ng of starting gDNA, TSCA is amenable to a wide range of samples, even those available in limited quantity. With the ability to generate up to 1,536 amplicons per reaction and integrated indices to support up to 96 samples per MiSeq® run, TSCA provides an unprecedented level of sample multiplexing. DesignStudio supports TSCA designs with a range of amplicon sizes optimized for different sample types (e.g. formalin-fixed paraffin-embedded) and reference genomes (e.g. human, mouse, rat, and bovine). Entire projects can now be accomplished in only days, leveraging the long paired-end–read capability, speed, and high data quality of the MiSeq system. 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