(头 ],,,,,,,,,, FileFormatVersion 2 ,,,,,,,,, RunName, V2_SampleSheet ,,,,,,,,, InstrumentType NovaSeq 6000 ,,,,,,,,, InstrumentPlatform, NovaSeq ,,,,,,,,, 化学、v1.5 ,,,,,,,,, 工作流,XP ,,,,,,,,, [ 读取 ],,,,,,,,,, Read1Cycles 151 ,,,,,,,,, Read2Cycles 151 ,,,,,,,,, Index1Cycles 8 ,,,,,,,,, Index2Cycles 8 ,,,,,,,,, [ TSO500L_Settings ],,,,,,,,,,AdapterRead1,AGATCGGAAGAGCACACGTCTGAACTCCAGTCA，，，，，，，，， AdapterRead2,AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT，，，，，，，，， AdapterBehavior,trim，，，，，，，，， MinimumTrimmedReadLength,35，，，，，，，，， MaskShortReads,35，，，，，，，，， overrideccles,U7N1Y143;I8;I8Sample_ID、Sample_Name Sample_Plate、Sample_Well Index_ID,指数,I7_Index_ID, index2, I5_Index_ID, Sample_Type,莱恩Test_Sample_1…UP01, TCCGGAGA, D702, AGGATAGG, D503, DNA, 1 Test_Sample_2…UP02, CTGAAGCT, D707, TCAGAGCC, D504, DNA, 1 Test_Sample_3…UP03, CGTAGCTC, D717, CATCCGAA, D509, DNA, 1 Test_Sample_4…UP04, GAATTCGT, D706, TTATGAGT, D510, DNA, 1Test_Sample_5…UP05、AGCGATAG D712、ACGAATAA D513, DNA, 1 Test_Sample_6…UP06, GCGATTAA, D724, GATCTGCT, D515, DNA, 1 Test_Sample_7…UP07, ATTCAGAA, D705, AGGCTATA, D501, DNA, 2 Test_Sample_8…UP08, GAATAATC, D713, GCCTCTAT, D502, DNA, 2 Test_Sample_9…UP09, TTAATCAG, D715, CTTCGCCT, D505, DNA, 2 Test_Sample_10…表白,CGCTCATT, D703, TAAGATTA, D506, DNA, 2Test_Sample_11…皮层,TCCGCGAA、D710 AGTAAGTA, D517, DNA, 2 Test_Sample_12…UP12 ATTACTCG, D701, GACTTCCT, D518, DNA, 2 Test_Sample_13…UP13 ACTGCTTA, D716, AGAGGCGC, D511, DNA, 3 Test_Sample_14…UP14 ATGCGGCT, D714, TAGCCGCG, D512, DNA, 3 Test_Sample_15…UP15 GCCTCTCT, D718, TTCGTAGG, D514, DNA, 3 Test_Sample_16…UP16 GCCGTAGG, D719 CGCTCCGC D516, DNA, 3Test_Sample_17…UP01、TCCGGAGA D702、AGGATAGG D503, DNA, 3 Test_Sample_18…UP02 CTGAAGCT, D707, TCAGAGCC, D504, DNA, 3 Test_Sample_19…UP03 CGTAGCTC, D717, CATCCGAA, D509, DNA, 4 Test_Sample_20…UP04 GAATTCGT, D706, TTATGAGT, D510, DNA, 4 Test_Sample_21…UP05 AGCGATAG, D712, ACGAATAA, D513, DNA, 4 Test_Sample_22…UP06 GCGATTAA, D724 GATCTGCT D515, DNA, 4Test_Sample_23…UP07、ATTCAGAA D705、AGGCTATA D501, DNA, 4 Test_Sample_24…UP08 GAATAATC, D713, [BCLConvert_Settings GCCTCTAT D502, DNA, 4 ],,,,,,,,,, SoftwareVersion, 3.6.3 ,,,,,,,,, AdapterRead1, AGATCGGAAGAGCACACGTCTGAACTCCAGTCA ,,,,,,,,, AdapterRead2, AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT ,,,,,,,,, AdapterBehavior,修剪……MinimumTrimmedReadLength 35 ,,,,,,,,, MaskShortReads 35 ,,,,,,,,, OverrideCycles U7N1Y143; I8; I8 U7N1Y143 ,,,,,,,,, [ BCLConvert_Data ],,,,,,,,,, Sample_ID、索引、index2巷……Test_Sample_1, TCCGGAGA, AGGATAGG, 1……Test_Sample_2 CTGAAGCT, TCAGAGCC, 1……Test_Sample_3 CGTAGCTC, CATCCGAA, 1……Test_Sample_4 GAATTCGT TTATGAGT 1……Test_Sample_5, AGCGATAG ACGAATAA 1……Test_Sample_6, GCGATTAA, GATCTGCT, 1……Test_Sample_7 ATTCAGAA, AGGCTATA, 2……Test_Sample_8 GAATAATC, GCCTCTAT, 2……Test_Sample_9 TTAATCAG, CTTCGCCT, 2……Test_Sample_10 CGCTCATT, TAAGATTA, 2……Test_Sample_11 TCCGCGAA, AGTAAGTA, 2……Test_Sample_12 ATTACTCG GACTTCCT 2……Test_Sample_13, ACTGCTTA AGAGGCGC 3……Test_Sample_14, ATGCGGCT, TAGCCGCG、3……Test_Sample_15 GCCTCTCT, TTCGTAGG、3……Test_Sample_16 GCCGTAGG, CGCTCCGC、3……Test_Sample_17 TCCGGAGA, AGGATAGG、3……Test_Sample_18 CTGAAGCT, TCAGAGCC、3……Test_Sample_19 CGTAGCTC, CATCCGAA, 4……Test_Sample_20, GAATTCGT TTATGAGT 4…… Test_Sample_21,AGCGATAG,ACGAATAA,4,,,,,,, Test_Sample_22,GCGATTAA,GATCTGCT,4,,,,,,, Test_Sample_23,ATTCAGAA,AGGCTATA,4,,,,,,, Test_Sample_24,GAATAATC,GCCTCTAT,4,,,,,,,