TruSight Myeloid Sequencing Panel
The TruSight Myeloid Sequencing Panel contains the following components and is shipped on dry ice unless specified otherwise below. Store the kit components at the specified temperature in designated pre-amplification and post-amplification areas.
Box 1, Pre-Amp
| Reagent |
Description |
Storage |
| ACD1 |
Amplicon Control DNA 1 |
-25°C to -15°C |
| ACP1 |
Amplicon Control Oligo Pool 1 |
-25°C to -15°C |
| OHS2 |
Oligo Hybridization for Sequencing Reagent 2 |
-25°C to -15°C |
| ELM4 |
Extension Ligation Mix 4 |
-25°C to -15°C |
| PMM2 |
PCR Master Mix 2 |
-25°C to -15°C |
| TDP1 |
TruSeq DNA Polymerase 1 |
-25°C to -15°C |
| SW1 |
Stringent Wash 1 |
2°C to 8°C |
| UB1 |
Universal Buffer 1 |
2°C to 8°C |
|
Barcode plate stickers for HYP, FPU, IAP |
Room temperature |
Box 2, Pre-Amp
This box is shipped at room temperature. As soon as you receive your kit, remove LNB1 and store at 2°C to 8°C in the post-amplification area. The filter plate should remain in the pre-amplification area at room temperature.
| Reagent |
Description |
Storage |
| LNB1 |
Library Normalization Beads 1 |
2°C to 8°C |
|
Filter plate with lid |
Room temperature |
Box 3, Post-Amp
| Reagent |
Description |
Storage |
| HT1 |
Hybridization Buffer |
-25°C to -15°C |
| LNA1 |
Library Normalization Additives 1 |
-25°C to -15°C |
| LNW1 |
Library Normalization Wash 1 |
2°C to 8°C |
| LNS2 |
Library Normalization Storage Buffer 2 |
Room temperature |
| EBT |
Elution Buffer with Tris |
Room temperature |
|
Barcode plate stickers for CLP, LNP, SGP, PAL, DAL |
Room temperature |
Box 4, TruSight Myeloid Custom Amplicon Oligos Box
| Reagent |
Description |
Storage |
| TSO |
TruSight Oligo Tube |
-25°C to -15°C |