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| NextSeq 550 System | NextSeq 2000 System Run 1 | NextSeq 2000 System Run 2 | |
|---|---|---|---|
| Primary run metrics | |||
| Paired-end reads PF | 514.2M | 518.3M | 532.8M |
| Yield | 103.9 Gb | 103.7 Gb | 106.5 Gb |
| Error rate | 0.34% | 0.32% | 0.30% |
| 二次分析指标 | |||
| Autosome callability | 95.61% | 95.95% | 95.94% |
| % aligned reads | 99.15% | 99.08% | 99.07% |
| Mean target coverage depth | 78.05 | 77.85 | 78.15 |
| Uniformity of coverage | 93.20% | 93.23% | 93.28% |
| SNV precision | 99.3% | 99.3% | 99.33% |
| SNV recall | 94.1% | 94.2% | 94.27% |
| Indel precision | 88.0% | 89.3% | 89.19% |
| Indel recall | 83.3% | 84.7% | 84.75% |
| NextSeq 550 System | NextSeq 2000 System Run 1 | NextSeq 2000 System Run 2 | |
|---|---|---|---|
| Metric | |||
| Paired-end reads PF | 422.4M | 511.6M | 523.7M |
| Yield | 63.6 Gb | 76.76 Gb | 78.25 Gb |
| Error rate | 0.27% | 0.27% | 0.33% |
| Mean no. of genes detected (n = 24) | 24,355 | 24,284 | 24,357 |
Quantification of specific RNA targets by bulk mRNA-Seq using the NextSeq 550 System are plotted on the y-axis. Results using the NextSeq 2000 System are plotted on the x-axis. Concordance of data is observed along the y = x trendline (R2> 0.99 across four replicates).
| Read length | NextSeq 1000/2000 P1 Reagents | NextSeq 1000/2000 P2 Reagents | NextSeq 2000 P3 Reagents |
|---|---|---|---|
| Output per flow cella | |||
| 1 × 50 bp (P3 only) | 60 Gb | ||
| 2 × 50 bp | 40 Gb | 120 Gb | |
| 2 × 100 bp | 80 Gb | 240 Gb | |
| 2 × 150 bp | 30 Gb | 120 Gb | 360 Gb |
| Reads CPF | 100M | 400M | 1.2B |
| Quality scoresb | |||
| 1 × 50 bp | ≥ 90% of bases higher than Q30 | ||
| 2 × 50 bp | ≥ 90% of bases higher than Q30 | ||
| 2 × 100 bp | ≥ 85% of bases higher than Q30 | ||
| 2 × 150 bp | ≥ 85% of bases higher than Q30 | ||
| Run time | |||
| 1 × 50 bp | ~11 hours | ||
| 2 × 50 bp | ~13 hours | ~19 hours | |
| 2 × 100 bp | ~21 hours | ~33 hours | |
| 2 × 150 bp | ~19 hours | ~29 hours | ~48 hours |
Technical Note | PDF |3 versions
Resolve the whole transcriptome within tissue architecture
Technical Note | PDF3 MB
NextSeq 2000 Exome Sequencing Solution
Application Note | PDF< 1 MB
High-resolution, high-throughput spatial transcriptomics of complex tissues
Application Note | PDF3 MB
NextSeq™ 1000 and NextSeq 2000 Sequencing Systems Specifications Sheet
Data Sheet | PDF< 1 MB
NextSeq 1000/2000 Sequencing System Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions
All NextSeq 1000/2000 Sequencing System Support Resources Support
NextSeq 1000/2000 reagent kits contain a reagent cartridge, flow cell, and resuspension buffer. Multiple flow cell configurations offer adjustable output based on project needs.
Achieve rapid, targeted interrogation of an expansive number of target genes with exceptional capture efficiency and coverage uniformity.
Highly sensitive detection and characterization of common respiratory viruses, including COVID-19 strains, with comprehensive, rapid target enrichment sequencing.
Provides a reproducible, economical solution for sequencing RNA from FFPE tissues and other low-quality samples. Accuracy from as little as 10 ng total RNA.
A fast, integrated workflow for a wide range of applications providing targeted resequencing for custom panels, fixed panels, and whole-exome enrichment.