Illumina DNA PCR-Free Prep Training
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Illumina公司DNA PCR-Free图书馆提供flexibil做好准备ity for many whole-genome sequencing applications.
Save Time and Resources
The Illumina DNA PCR-Free Library Prep Kit uses a fast, user-friendly workflow. On-Bead Tagmentation chemistry reduces total library prep time to ~1.5 hours, from DNA extraction to library normalization.
Simplify Lab Operations
The Illumina DNA PCR-Free workflow supports a broad DNA input range (25 ng to 300 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots.
You can use liquid-handling robots to automate workflows for minimal touch points and significant time savings.
Obtain Reliable Results
While accommodating various study requirements, the Illumina DNA PCR-Free workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, delivering accurate base calling and variant identification for a broad range of DNA input amounts.
Access Flexible Throughput Options
The IDT for Illumina - DNA/RNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell. (Sets A and B are available now; Sets C and D are coming soon.) These unique dual index codes use 10 base pair codes. This change in base pair index codes require adjustments to the sequencing run setup.
Illumina DNA PCR-Free Prep | TruSeq DNA PCR-Free | TruSeq DNA Nano | Illumina DNA Prep | |
---|---|---|---|---|
Assay Time | ~ 1.5小时 | 5 hours total assay time | ~6 hours total assay time | ~3-4 hours (from DNA extraction to normalized library) |
Automation Capability | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots | Liquid Handling Robots |
Input Quantity | 25 ng to 300 ng | 1 ug DNA | 100 ng genomic DNA | Small genomes (e.g. microbial): 1-500 ng DNA. Large genomes (e.g. human): 100-500 ng DNA. (For blood and saliva, see the reference guide). |
Mechanism of Action | Bead-linked transposome | Mechanical DNA fragmentation and adapter ligation. Workflow is PCR-free and gel-free. | Mechanical DNA fragmentation and adapter ligation. Workflow uses reduced-bias PCR and is gel-free. | Bead-linked transposome |
Method | De Novo Sequencing,Whole-Genome Sequencing | Genotyping by Sequencing,Shotgun Sequencing,Whole-Genome Sequencing | Genotyping by Sequencing,Shotgun Sequencing,Whole-Genome Sequencing | Amplicon Sequencing,De Novo Sequencing,Shotgun Sequencing,Whole-Genome Sequencing |
Multiplexing | Up to 384-plex | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 24 single, 96 combinatorial (CD) dual, 24 unique dual and 96 unique dual (UD) combinations (available soon) | Up to 384 unique dual (UD) combinations and 96 combinatorial dual (CD) combinations |
Nucleic Acid Type | DNA | DNA | DNA | DNA |
Specialized Sample Types | Blood, Low-Input Samples, Not FFPE-Compatible, Saliva | Not FFPE-Compatible | Low-Input Samples, Not FFPE-Compatible | Blood, Not FFPE-Validated, Saliva |
Microbial whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF< 1 MB
High-performance whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF< 1 MB
Tunable insert sizes with Illumina DNA PCR-Free Prep, Tagmentation
Application Note | PDF< 1 MB
Optimal loading concentrations for Illumina DNA PCR-Free libraries
Technical Note | PDF< 1 MB
平衡样本有限公司verage for whole-genome sequencing
Technical Note | PDF< 1 MB
Illumina library preparation solutions
Brochure | PDF2 MB
Data Sheet | PDF |5 versions
Illumina DNA PCR-Free Prep index correction
product_file | CSV< 1 MB
NovaSeq Series Custom Primers Guide Documentation
Illumina Adapter Sequences Documentation
Custom Protocol Selector
Generates customized, end-to-end instructions