Key Applications and Methods
NovaSeq执行全基因组测序更有效率ciently and cost-effectively than ever. Its scalable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with simple streamlined automated workflows. Configure the system to sequence a trio in one day or up to 48 genomes in ~2 days for the most comprehensive coverage.
Prep
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing.
llumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 200x coverage/sample
DRAGEN Germline Pipeline (on BSSH or on DRAGEN server)
The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both.
DRAGEN Somatic Pipeline (on BSSH or on DRAGEN server)
The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF.
Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq System offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell.
Prep
*2.5 days total prep time, 6 hours hands-on time
Illumina DNA Prep with Enrichment
This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 100x coverage/sample
DRAGEN Enrichment
This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both.
Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. With the NovaSeq System, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell.
Prep
Illumina Stranded Total RNA Prep
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
Sequence
NovaSeq Reagent Kits v1.5
New kits feature updated reagent formulations with 2x longer shelf life and improved sequencing costs to help power your most impactful studies.
Analyze
*Time per sample, assuming 200x coverage/sample
DRAGEN RNA Pipeline
Performs alignment, quantification, and fusion detection.
Introduction to the NovaSeq 6000 System Workflow
Join Brian Steffy, Senior Lab Manager and David Miller, Manager of Sequencing Systems to learn about the streamlined workflow of the NovaSeq System. See first hand why this system is truly our most advanced high-throughput sequencer.
View Video
Featured NovaSeq Stories
New Liquid Biopsy and High-Throughput Assays Join TruSight Oncology Portfolio
The first liquid biopsy solution for detecting cancer biomarkers on the NovaSeq 6000 System is the latest addition to our portfolio of comprehensive cancer profiling assays.
Read Article
The Time is Now for Microbiome Studies
Dissecting host—microbial relationships with the NovaSeq 6000 System gives researchers and pharmaceutical companies data to refine drug discovery.
Read Interview
Uncovering the Intracellular Communication of Tumors
Using the NovaSeq 6000 System, scientists are looking at the microenvironment surrounding tumors. Understanding this environment could tell us more about how cancer cells migrate and become drug resistant.
Read InterviewMore Applications and Methods
Target Enrichment
Use sequence-specific hybridization to analyze genomic regions of interest.
Coding Transcriptome Analysis
Detect both known and novel features of the coding transcriptome using sequence-specific capture of RNA coding regions.
Targeted DNA Sequencing
Targeted resequencing focuses time, expenses, and analysis on sequencing only a subset of genes or genome regions of research interest.
Methylation Sequencing
Enhance epigenetic studies with high-coverage density and flexibility enabled by sequencing-based DNA methylation analysis.
Gene Expression Profiling with mRNA-Seq
mRNA sequencing (mRNA-Seq) detects known and novel transcripts and measures transcript abundance for accurate, comprehensive expression profiling.
De NovoSequencing
De novosequencing refers to sequencing a novel genome with no reference sequence available. NGS enables fast, accurate characterization of any species.
DNA-Protein Interaction Analysis with ChIP-Seq
Combining chromatin immunoprecipitation (ChIP) assays with sequencing, ChIP-Seq is a powerful method for genome-wide surveys of gene regulation.
Population Studies
Robust, secure, and scalable platforms to aggregate and interpret large-scale genomic data for population sequencing applications.
Metagenomics
Comprehensively sample genes in organisms present in a complex sample to evaluate bacterial diversity and detect unculturable microorganisms.
Single-Cell RNA Sequencing
Assess the individual contributions of single cells in complex tissues by profiling the transcriptome.
ctDNA Sequencing
NGS offers the sensitivity and specificity that cancer researchers need to detect low levels of ctDNA in the bloodstream.
RNA Drug Response Biomarker Discovery
Find out how to utilize RNA-Seq to discover and profile RNA-based drug response biomarkers. Access resources designed to help new users adopt this application.
Illumina Methods Guide
Access the information you need—from BeadChips to library preparation for genome, transcriptome, or epigenome studies to sequencer selection, analysis, and support—all in one place. Select the best tools for your lab with our comprehensive guide designed specifically for research applications.
Access GuideRelated Solutions
Cancer Genomics Research
Our sequencing and microarray technologies support a broad range of cancer genomics research applications, from DNA to RNA analysis, epigenetics, and more.
Microbial Genomics
Next-generation sequencing (NGS) is changing microbial genomics. Use NGS to discover novel microbes, monitor outbreaks, analyze food sources, and more.
Complex and Genetic Disease Research
Comprehensive array and next-generation sequencing solutions to accelerate research of various genetic complex diseases.